Article
Use of self-sustained sequence replication amplification reaction to analyze and detect mutations in zidovudine-resistant human immunodeficiency virus.
The Journal of infectious diseases - 1 Dec 1991
Gingeras T R, Prodanovich P, Latimer T, Guatelli J C, Richman D D, Barringer K J
Abstract excerpt
Mutations at amino acid positions 67, 70, 215, and 219 in the human immunodeficiency virus type 1 (HIV-1) pol gene correlate with the emergence of resistance to zidovudine (AZT). These four positions were monitored in viral RNA extracted from infected peripheral blood mononuclear cells (PBMC) and...
Topics
- Amino Acid Sequence
- Base Sequence
- Blotting, Southern
- DNA Replication
- Drug Resistance, Microbial
- Genetic Vectors
- Genotype
- HIV Infections
- HIV-1
- Humans
- Molecular Sequence Data
- Mutation
- Nucleic Acid Hybridization
- Oligonucleotide Probes
- Plasmids