Article
Expression of Hepatitis B virus surface antigen (HBsAg) from genotypes A, D and F and influence of amino acid variations related or not to genotypes on HBsAg detection.
The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases - 1 Aug 2009
Araujo Natalia M, Vianna Carlos O A, Moraes Marcia T B, Gomes Selma A
Abstract excerpt
The impact of hepatitis B virus (HBV) genotypes on the sensitivity of surface antigen (HBsAg) detection assays has been poorly investigated. Here, plasmids carrying consensus or variant coding sequences for HBV surface proteins from genotypes A, D and F, were constructed. HBsAg levels were evaluated in medium and extracts of transfected CHO cells by a commercial polyclonal-based assay. We show that HBsAg...
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