Article
Mutational analysis of arsonate binding by a CRIA+ antibody. VH and VL junctional diversity are essential for binding activity.
The Journal of biological chemistry - 25 Apr 1991
Hasemann C A, Capra J D
Abstract excerpt
To assess the impact of various heavy and light chain mutations on p-azophenylarsonate binding, murine antibodies have been produced in insect cells (SF9) utilizing a baculovirus expression system. When expressed in this system, an antibody composed of a canonical CRIA+ heavy and light chain can...
Topics
- Amino Acid Sequence
- Animals
- Baculoviridae
- Base Sequence
- Gene Expression Regulation, Viral
- Genes, Viral
- Genetic Vectors
- Glycosylation
- Hybridomas
- Immunoglobulin G
- Immunoglobulin Variable Region
- Insecta
- Mice
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Transfection
- p-Azobenzenearsonate