Article
Clustered H chain somatic mutations shared by anti-p-azophenylarsonate antibodies confer enhanced affinity and ablate the cross-reactive idiotype.
Journal of immunology (Baltimore, Md. : 1950) - 1 Oct 1990
Parhami-Seren B, Wysocki L J, Margolies M N, Sharon J
Abstract excerpt
A cluster of four consecutive CDR2 somatic mutations are shared by the VH regions of two independently isolated hybridoma antibodies with specificity for p-azophenylarsonate (Ars). The mutations appear to be derived by a series of independent events. To assess the influence of these shared somati...
Topics
- Amino Acid Sequence
- Animals
- Antibodies, Monoclonal
- Antibody Affinity
- Azo Compounds
- Base Sequence
- Cross Reactions
- DNA Mutational Analysis
- Genes, Immunoglobulin
- Hybridomas
- Immunoglobulin Heavy Chains
- Immunoglobulin Idiotypes
- Mice
- Molecular Sequence Data
- Mutation
- Oligonucleotides
- Transfection
- p-Azobenzenearsonate