Generation and analysis of random point mutations in an antibody CDR2 sequence: many mutated antibodies lose their ability to bind antigen.

doi:10.1084/jem.176.3.855

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Article

Generation and analysis of random point mutations in an antibody CDR2 sequence: many mutated antibodies lose their ability to bind antigen.

The Journal of experimental medicine - 1 Sept 1992

Chen C, Roberts V A, Rittenberg M B

Abstract excerpt

We have investigated the impact of mutations on the binding functions of the phosphocholine (PC)-specific T15 antibody in the absence of antigen selection pressure. The H chain complementarity determining region 2 (CDR2) sequence of T15 antibody was saturated with point mutations by in vitro rand...

Topics

Amino Acid Sequence
Antigens
Base Sequence
DNA
Immunoglobulin Heavy Chains
Immunoglobulin Variable Region
Molecular Sequence Data
Mutation
Phosphorylcholine
Transformation, Genetic

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