Article
The molecular and biochemical characterization of mutant monoclonal antibodies with increased antigen binding.
Journal of immunology (Baltimore, Md. : 1950) - 15 Mar 1991
French D L, Pollock R R, Aguila H L, Scharff M D
Abstract excerpt
Mutant mAb with increased Ag binding were generated from a hybridoma cell line, 36-65, that secretes an IgG1,kappa anti-p-azophenylarsonate-(Ars) specific antibody. The mutant antibodies were identified using an Ars-specific ELISA and sib selection so that approximately 10(6) cells could be analy...
Topics
- Amino Acid Sequence
- Animals
- Antibodies, Monoclonal
- Antibody Affinity
- Base Sequence
- Electrophoresis, Polyacrylamide Gel
- Enzyme-Linked Immunosorbent Assay
- Immunoglobulin Constant Regions
- Immunoglobulin Heavy Chains
- Mice
- Molecular Sequence Data
- Molecular Weight
- Mutation
- Poly A
- Polymers
- RNA, Messenger
- p-Azobenzenearsonate