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Article

Evaluation of loss and change of specificity resulting from random mutagenesis of an antibody VH region.

Journal of immunology (Baltimore, Md. : 1950) - 15 Dec 1995

Casson L P, Manser T

Abstract excerpt

Most data available from in vivo sources regarding the impact of somatic hypermutation on Ab V region structure and function are heavily biased due to the influence of clonal selection. In an effort to address this issue directly, we "randomly" introduced point mutations throughout the length of...

Topics

Amino Acid Sequence
Animals
Antibody Affinity
Cloning, Molecular
Gene Library
Genetic Vectors
Immunoglobulin Heavy Chains
Immunoglobulin Variable Region
Mice
Molecular Sequence Data
Mutagenesis
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Polymerase Chain Reaction

Recombinant Fusion Proteins

p-Azobenzenearsonate

Source

Identifiers

PMID: 7499849

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-7499849-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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