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Article

Structural correlates of high antibody affinity: three engineered amino acid substitutions can increase the affinity of an anti-p-azophenylarsonate antibody 200-fold.

Proceedings of the National Academy of Sciences of the United States of America - 1 Jun 1990

Sharon J

Abstract excerpt

The basis for the 200-fold difference in affinity between two hybridoma antibodies specific for the hapten p-azophenylarsonate (Ars) that have diversified by somatic hypermutation was examined. Oligonucleotide-directed mutagenesis was used to sequentially convert the nucleotide sequence of the lower-affinity antibody into that of the higher-affinity one, and the mutant antibodies generated by transfection of...

Topics

Amino Acid Sequence
Animals
Antibodies
Antibody Affinity
Azo Compounds
Base Sequence
Genes, Immunoglobulin
Haptens
Immunoglobulin Variable Region
Mice
Mice, Inbred A

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutation

Oligonucleotide Probes

Transfection

p-Azobenzenearsonate

Source

Open in PubMed Open full text

Identifiers

PMID: 2352950
DOI: 10.1073/pnas.87.12.4814
PMCID: PMC54208

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2352950-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Structural correlates of high antibody affinity: three engineered amino acid substitutions can increase the affinity of an anti-p-azophenylarsonate antibody 200-fold.PMID 2352950Read ↗

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