Structure of Escherichia coli K-12 miaA and characterization of the mutator phenotype caused by miaA insertion mutations.

Abstract excerpt

Previously, we reported several unusual relationships between the 2-methylthio-N6-(delta 2-isopentenyl)adenosine-37 (ms2i6A-37) tRNA modification and spontaneous mutagenesis in Escherichia coli K-12 (D. M. Connolly and M. E. Winkler, J. Bacteriol. 171:3233-3246, 1989). To confirm and extend these observations, we determined the structure of miaA, which mediates the first step of ms2i6A-37 synthesis, and...

Topics

• Amino Acid Sequence
• Base Sequence
• DNA, Bacterial
• Escherichia coli
• Isopentenyladenosine
• Molecular Sequence Data
• Mutagenesis
• Mutagenesis, Insertional
• Nucleic Acid Conformation
• Open Reading Frames
• Phenotype