Article
Analysis of apolipoprotein E genotypes by the Amplification Refractory Mutation System.
Clinical chemistry - 1 Feb 1991
Wenham P R, Newton C R, Price W H
Abstract excerpt
The Amplification Refractory Mutation System (ARMS) has been successfully applied to the detection of apolipoprotein (apo) E genotypes in human DNA extracted from peripheral blood. By using four allele-specific oligonucleotide primers and one common primer, one can identify the three common alleles of the apo E genetic polymorphism, epsilon 2, epsilon 3, and epsilon 4. The system amplifies two sequences of the...
Topics
- Alleles
- Apolipoproteins E
- Base Sequence
- DNA
- Electrophoresis, Agar Gel
- Gene Amplification
- Genotype
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Genetic