Article
Rapid identification of the common apo E isoform genotype using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP).
Molecular and cellular probes - 1 Feb 1994
Aozaki R, Kawaguchi R, Ogasa U, Hikiji K, Kubo N, Sakurabayashi I
Abstract excerpt
Polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) was used to identify the common apoprotein E (apo E) isoform genotypes. DNA from the target sequence on apo E was amplified by PCR from peripheral blood genomic DNA. Non-radiolabelled natural PCR products were electrophoresed on a polyacrylamide gradient gel and automatically silver-stained. Bands compatible with each of the three common...
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