Article
Rapid identification of the common apo E isoform genotype using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP).
Molecular and cellular probes - 1 Feb 1994
Aozaki R, Kawaguchi R, Ogasa U, Hikiji K, Kubo N, Sakurabayashi I
Abstract excerpt
Polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) was used to identify the common apoprotein E (apo E) isoform genotypes. DNA from the target sequence on apo E was amplified by PCR from peripheral blood genomic DNA. Non-radiolabelled natural PCR products were electropho...
Topics
- Apolipoproteins E
- Base Sequence
- DNA, Single-Stranded
- Genotype
- Humans
- Isomerism
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Polymorphism, Restriction Fragment Length