Article
Apolipoprotein E genotyping by multiplex tetra-primer amplification refractory mutation system PCR in single reaction tube.
Journal of biotechnology - 31 Aug 2007
Yang Young Geun, Kim Jong Yeol, Park Su Jeong, Kim Suhng Wook, Jeon Ok-Hee, Kim Doo-Sik
Abstract excerpt
Apolipoprotein E (APOE) plays a critical role in lipoprotein metabolism by binding to both low-density lipoprotein and APOE receptors. The APOE gene has three allelic forms, epsilon2, epsilon3, and epsilon4, which encode different isoforms of the APOE protein. In this study, we have developed a new genotyping method for APOE. Our multiplex tetra-primer amplification refractory mutation system (multiplex T-ARMS)...
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