Apolipoprotein E genotyping by capillary electrophoretic analysis of restriction fragments.

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Article

Apolipoprotein E genotyping by capillary electrophoretic analysis of restriction fragments.

Clinical chemistry - 1 Aug 1997

de Bellis G, Salani G, Panigone S, Betti F, Invernizzi L, Luzzana M

Abstract excerpt

We present the genotyping of apolipoprotein (apo) E by means of restriction fragment analysis of amplified genomic DNA by high-performance capillary electrophoresis and a replaceable non-gel-sieving matrix. This procedure streamlines the genotyping of apo E in large-scale population studies because of the automation and speed of capillary electrophoresis.

Topics

Apolipoproteins E
Codon
DNA
Deoxyribonucleases, Type II Site-Specific
Electrophoresis, Capillary
Electrophoresis, Polyacrylamide Gel
Genotype
Humans
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
Reproducibility of Results
Restriction Mapping
Sensitivity and Specificity

Source

Open in PubMed

Identifiers

PMID: 9267308

Provenance

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