Apolipoprotein E2/E3/E4 genotyping with agarose gels.

Article

Apolipoprotein E2/E3/E4 genotyping with agarose gels.

Clinical chemistry - 1 Jan 1995

Dallinga-Thie G M, van Linde-Sibenius Trip M, Kock L A, De Bruin T W

Abstract excerpt

Here we report an improved, simple method to assign the human apolipoprotein (apo) E genotype and its isoforms, apo E2, apo E3, and apo E4. Genomic DNA was amplified with specific primers that included the polymorphic region of amino acids 112 and 158. Digestion of the product with Hhal resulted...

Topics

Apolipoprotein E2
Apolipoprotein E3
Apolipoprotein E4
Apolipoproteins E
Base Sequence
Electrophoresis, Agar Gel
Genotype
Humans
Isoelectric Focusing
Molecular Sequence Data
Phenotype
Polymerase Chain Reaction

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