Article
Genetic studies of human apolipoproteins: XIV. A simple agarose isoelectric focusing gel method for apolipoprotein E phenotyping.
Electrophoresis - 1 Apr 1990
Kamboh M I, Kelly L J, Ferrell R E
Abstract excerpt
A new and simplified procedure is described for apolipoprotein E (APO E) phenotyping from native plasma or serum samples. Diluted or dialyzed samples are separated on agarose isoelectric focusing gels followed by protein blotting on nitrocellulose membranes. APO E banding patterns are localized immunologically using polyclonal goat anti-APO E antiserum as the primary antibody and rabbit anti-goat IgG conjugated...
Topics
- Apolipoproteins E
- Gene Frequency
- Humans
- Hydrogen-Ion Concentration
- Immunoblotting
- Isoelectric Focusing
- Phenotype
- Polymorphism, Genetic
- United States
- White People
