Article
Rapid diagnosis of familial defective apolipoprotein B-100 by Amplification Refractory Mutation System.
Clinical chemistry - 1 Nov 1991
Wenham P R, Newton C R, Houlston R S, Price W H
Abstract excerpt
We report a method for the diagnosis of familial defective apolipoprotein (apo) B-100, using the Amplification Refractory Mutation System (ARMS) and either whole blood or extracted DNA in the polymerase chain reaction. Normal and mutant alleles are identified by using two allele-specific oligonuc...
Topics
- Apolipoprotein B-100
- Apolipoproteins B
- Base Sequence
- Diagnosis, Differential
- Dimethyl Sulfoxide
- Heterozygote
- Humans
- Hyperlipoproteinemia Type II
- Lipid Metabolism, Inborn Errors
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction