Article
Shuttle vector plasmid propagation in human peripheral blood lymphocytes facilitated by liposome-mediated transfection.
DNA and cell biology - 1 Jan 2000
Brant M, Nachmansson N, Norrman K, Regnell I, Bredberg A
Abstract excerpt
Transfection of human peripheral blood lymphocytes facilitated by a positively charged liposome preparation (Lipofectin, BRL) is 100-fold more efficient than the DEAE dextran technique for the uptake and replication of shuttle vector plasmid DNA. The yield of progeny plasmids obtained from 10 ml of blood was high enough for mutational analysis. A marked increase in the mutation frequency of the shuttle vector...
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