Shuttle vector plasmid propagation in human peripheral blood lymphocytes facilitated by liposome-mediated transfection.

doi:10.1089/dna.1991.10.75

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Article

Shuttle vector plasmid propagation in human peripheral blood lymphocytes facilitated by liposome-mediated transfection.

DNA and cell biology - 1 Jan 2000

Brant M, Nachmansson N, Norrman K, Regnell I, Bredberg A

Abstract excerpt

Transfection of human peripheral blood lymphocytes facilitated by a positively charged liposome preparation (Lipofectin, BRL) is 100-fold more efficient than the DEAE dextran technique for the uptake and replication of shuttle vector plasmid DNA. The yield of progeny plasmids obtained from 10 ml...

Topics

DNA Repair
Genetic Vectors
Humans
Liposomes
Lymphocytes
Mutation
Plasmids
Transfection

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