Article
Dependence of the mutation spectrum in a shuttle plasmid replicated in human lymphoblasts on dose of gamma radiation.
International journal of radiation biology - 1 May 1991
Sikpi M O, Freedman M L, Ziobron E R, Upholt W B, Lurie A G
Abstract excerpt
The frequencies and types of mutations induced in the target gene, supF-tRNA, of the shuttle vector pZ189 were analysed following the replication of the gamma-irradiated plasmid in the human lymphoblastoid cell line, GM606. The mutation frequency measured in progeny of unirradiated pZ189 was 1.02 x 10(-4), increasing to 17.5 x 10(-4) at 1000 cGy, and to 63.4 x 10(-4) at 5000 cGy, approximately 17- and 62-fold...
Topics
- Cell Line
- Cesium Radioisotopes
- DNA Replication
- Dose-Response Relationship, Radiation
- Gamma Rays
- Genetic Vectors
- Humans
- In Vitro Techniques
- Mutation
- Plasmids
- Radiation Genetics