Article
[Construction of an EB virus based shuttle vector plasmid pZH32].
Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae - 1 Dec 1992
Zhou X
Abstract excerpt
The shuttle vector can replicate in both bacteria and eukaryotic cells. We have constructed a shuttle vector pZH32 based on the EB virus. Three component parts of the plasmid come from pSV2gpt, pS189 and pMCi5. An E. coli tyrosine suppressor tRNA gene, supF, was used as a mutagenic target in this plasmid, and a gpt gene was used as a selectable marker gene for transformed cells. This pZH32 based on EBV replicates...
Topics
- DNA, Recombinant
- Escherichia coli
- Genes, Bacterial
- Genetic Vectors
- Herpesvirus 4, Human
- Mutation
- Plasmids