Article
Analysis of N-methyl-N-nitrosourea-induced mutations in a shuttle vector plasmid propagated in mouse O6-methylguanine-DNA methyltransferase-deficient cells in comparison with proficient cells.
Cancer research - 1 Dec 1991
Moriwaki S, Yagi T, Nishigori C, Imamura S, Takebe H
Abstract excerpt
A shuttle vector plasmid, pYZ289, was constructed from the pZ189 plasmid and polyoma virus DNA. The plasmid contains a supF gene as a marker of mutation and can replicate in both Escherichia coli and mouse cells. The pYZ289 plasmids treated with N-methyl-N-nitrosourea were passed through mouse ce...
Topics
- Animals
- Base Sequence
- Cell Line, Transformed
- Dose-Response Relationship, Drug
- Genes, Viral
- Methylnitrosourea
- Methyltransferases
- Mice
- Mice, Hairless
- Molecular Sequence Data
- Mutation
- O(6)-Methylguanine-DNA Methyltransferase
- Plasmids
- Specific Pathogen-Free Organisms
- Transfection