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Article

Conditional and reversible disruption of essential herpesvirus proteins.

Nature methods - 1 Aug 2009

Glass Mandy, Busche Andreas, Wagner Karen, Messerle Martin, Borst Eva Maria

Abstract excerpt

Elucidating the function of essential proteins of complex pathogenic viruses is impeded by a paucity of complementing systems. By fusing a destabilizing domain of the FK506-binding protein to essential cytomegalovirus proteins, we generated virus mutants in which amounts of fusion proteins and viral growth can be regulated by the synthetic ligand shield-1. This conditional approach will greatly facilitate the...

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Identifiers

PMID: 19578384
DOI: 10.1038/nmeth.1346

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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