Article
Conditional expression of foreign genes by temperature-sensitive mutants of vaccinia virus.
Gene - 15 Nov 1990
Chambers T M, Essani K, Webster R G
Abstract excerpt
To assess the utility of two temperature-sensitive (ts) mutant vaccinia viruses as vectors for the conditional in vitro expression of recombinant foreign genes, we have studied the kinetics of expression of foreign genes incorporated into these viruses. At nonpermissive temperature, 40 degrees C, these viruses were defective either in DNA synthesis or in virus assembly. Foreign gene expression was affected by the...
Topics
- DNA, Recombinant
- DNA, Viral
- Gene Expression Regulation
- Genetic Vectors
- Kinetics
- Mutation
- Phenotype
- Promoter Regions, Genetic
- Temperature
- Vaccinia virus