Regulated ectopic expression and allelic-replacement mutagenesis as a method for gene essentiality testing in Staphylococcus aureus.

doi:10.1006/plas.2001.1526

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Article

Regulated ectopic expression and allelic-replacement mutagenesis as a method for gene essentiality testing in Staphylococcus aureus.

Plasmid - 1 Jul 2001

Fan F, Lunsford R D, Sylvester D, Fan J, Celesnik H, Iordanescu S, Rosenberg M, McDevitt D

Abstract excerpt

Conditional expression systems were utilized for the ectopic induction of essential genes in Staphylococcus aureus. Resulting strains were then subjected to allelic-replacement mutagenesis of the native allele under inducing conditions for expression of the ectopic copy of the gene. This strategy produced test strains whereby cellular viability was uniquely dependent on the presence of inducer and provided a...

Topics

Alleles
Antiporters
Bacterial Proteins
Cytoskeletal Proteins
Gene Expression Regulation, Bacterial
Genes, Bacterial
Membrane Proteins
Mutagenesis
Serine Endopeptidases
Staphylococcus aureus

Source

Open in PubMed

Identifiers

PMID: 11535039
DOI: 10.1006/plas.2001.1526

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