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Article

Comprehensive mutational analysis of a herpesvirus gene in the viral genome context reveals a region essential for virus replication.

Journal of virology - 1 Aug 2004

Bubeck Anja, Wagner Markus, Ruzsics Zsolt, Lötzerich Mark, Iglesias Margot, Singh Ila R, Koszinowski Ulrich H

Abstract excerpt

Essential viral proteins perform vital functions during morphogenesis via a complex interaction with other viral and cellular gene products. Here, we present a novel approach to comprehensive mutagenesis of essential cytomegalovirus genes and biological analysis in the 230-kbp-genome context. A random Tn7-based mutagenesis procedure at the single-gene level was combined with site-specific recombination via the...

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Identifiers

PMID: 15254174
DOI: 10.1128/JVI.78.15.8026-8035.2004
PMCID: PMC446129

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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