Article
Generation of a set of conditional analog-sensitive alleles of essential protein kinases in the fission yeast Schizosaccharomyces pombe.
Cell cycle (Georgetown, Tex.) - 15 Oct 2011
Cipak Lubos, Zhang Chao, Kovacikova Ines, Rumpf Cornelia, Miadokova Eva, Shokat Kevan M, Gregan Juraj
Abstract excerpt
The genome of the fission yeast Schizosaccharomyces pombe encodes for 17 protein kinases that are essential for viability. Studies of the essential kinases often require the use of mutant strains carrying conditional alleles. To inactivate these kinases conditionally, we applied a recently developed chemical genetic strategy. The mutation of a single residue in the ATP-binding pocket confers sensitivity to...
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