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Article

A modified screening system for loss-of-function and dominant negative alleles of essential MCMV genes.

PloS one - 1 Jan 2014

Pogoda Madlen, Bosse Jens B, Conzelmann Karl-Klaus, Koszinowski Ulrich H, Ruzsics Zsolt

Abstract excerpt

Inactivation of gene products by dominant negative mutants is a valuable tool to assign functions to yet uncharacterized proteins, to map protein-protein interactions or to dissect physiological pathways. Detailed functional and structural knowledge about the target protein would allow the construction of inhibitory mutants by targeted mutagenesis. Yet, such data are limited for the majority of viral proteins, so...

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Identifiers

PMID: 24733555
DOI: 10.1371/journal.pone.0094918
PMCID: PMC3986410

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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