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Article

Construction of conditional knockdown mutants in mycobacteria.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015

Schnappinger Dirk, O'Brien Kathryn M, Ehrt Sabine

Abstract excerpt

By definition, essential genes are fundamental to bacterial growth, yet the functions of many such genes remain unknown. Essential genes furthermore are central to the activity of most antibacterial drugs and among the most attractive targets for the development of new therapeutics. This chapter describes how synthetic genetic switches that utilize transcriptional repression, controlled proteolysis, or both to...

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PMID: 25779315
DOI: 10.1007/978-1-4939-2450-9_9

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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