Article
High-resolution DNA melting analysis for simultaneous mutation scanning and genotyping in solution.
Clinical chemistry - 1 Oct 2005
Zhou Luming, Wang Lesi, Palais Robert, Pryor Robert, Wittwer Carl T
Abstract excerpt
BACKGROUND: High-resolution DNA melting analysis with saturation dyes for either mutation scanning of PCR products or genotyping with unlabeled probes has been reported. However, simultaneous PCR product scanning and probe genotyping in the same reaction has not been described. METHODS: Asymmetric PCR was performed in the presence of unlabeled oligonucleotide probes and a saturating fluorescent DNA dye....
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