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Article

Simultaneous mutation scanning and genotyping by high-resolution DNA melting analysis.

Nature protocols - 1 Jan 2007

Montgomery Jesse, Wittwer Carl T, Palais Robert, Zhou Luming

Abstract excerpt

This protocol permits the simultaneous mutation scanning and genotyping of PCR products by high-resolution DNA melting analysis. This is achieved using asymmetric PCR performed in the presence of a saturating fluorescent DNA dye and unlabeled oligonucleotide probes. Fluorescent melting curves of...

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Identifiers

PMID: 17401339
DOI: 10.1038/nprot.2007.10

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-17401339-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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