Article
Genotyping DNA Variants with High-Resolution Melting Analysis.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017
Vossen Rolf H A M
Abstract excerpt
High-resolution melting analysis (HRMA) is a simple, quick, and effective method to scan and screen PCR amplicons for sequence variants. HRMA is a nondestructive closed tube assay; after PCR, DNA melting can directly be performed on the amplified samples without any purification or separation steps. For single SNP genotyping, HRMA is an attractive alternative to Sanger sequencing, restriction enzyme analysis, and...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
