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Haplotype and Presence-Absence Variation Analysis Using High-Resolution Melting (HRM) Analysis for Genotyping Genes of Interest.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2025

Torres Anthony, Givens Robert, Gaudino Reginald

Abstract excerpt

This chapter outlines a basic protocol for DNA extraction, applicable to both FTA cards and leaf pieces. The extracted DNA then undergoes a pre-amplification enrichment reaction, followed by dilution and preparation for input into a real-time amplification with High-Resolution Melt (HRM) detection. The resulting melting genotypes are produced for comparison and analysis, providing a practical approach for...

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DNA, Plant
Genotype
Genotyping Techniques
Haplotypes
Nucleic Acid Denaturation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Real-Time Polymerase Chain Reaction

Transition Temperature

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PMID: 40580291
DOI: 10.1007/978-1-0716-4642-7_11

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