Article
Genotyping by high-resolution melting analysis.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014
Tucker Elise J, Huynh Bao Lam
Abstract excerpt
High-resolution melting (HRM) analysis is a simple, closed tube, post-PCR method used to identify genetic variation. The method is highly sensitive and can discriminate DNA sequence variants based on length (such as insertions or deletions), composition (such as single nucleotide polymorphisms, i.e., SNP) or strand complementarity (such as heterozygous or homozygous material). The technique involves PCR...
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