Article
Method for clone checking.
Electrophoresis - 1 Jun 2003
Nourizad Nader, Gharizadeh Baback, Nyrén Pål
Abstract excerpt
A new method for simple and fast clone checking is described. We combined the Pyrosequencing technology with a preprogrammed nucleotide dispensation strategy for fast analysis of DNA constructs. To test this method, the N-terminus region of plasmids constructed for the production of recombinant apyrase was analyzed. Of the ten plasmids tested, seven constructs were correct, two constructs showed one base...
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