Article
Low-fidelity Pyrococcus furiosus DNA polymerase mutants useful in error-prone PCR.
Nucleic acids research - 15 Dec 2004
Biles Benjamin D, Connolly Bernard A
Abstract excerpt
Random mutagenesis constitutes an important approach for identifying critical regions of proteins, studying structure-function relations and developing novel proteins with desired properties. Perhaps, the most popular method is the error-prone PCR, in which mistakes are introduced into a gene, and hence a protein, during DNA polymerase-catalysed amplification cycles. Unfortunately, the relatively high fidelities...
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