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Article

PCR performance of the highly thermostable proof-reading B-type DNA polymerase from Pyrococcus abyssi.

FEMS microbiology letters - 19 Nov 2002

Dietrich Jacques, Schmitt Philippe, Zieger Montserrat, Preve Brigitte, Rolland Jean-Luc, Chaabihi Hassan, Gueguen Yannick

Abstract excerpt

DNA polymerase from the archaeon Pyrococcus abyssi strain Orsay was expressed in Escherichia coli. The recombinant DNA polymerase (Pab) was purified to homogeneity by heat treatment followed by 5 steps of chromatography and characterized for PCR applications. Buffer optimization experiments indic...

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PMID: 12445650
DOI: 10.1111/j.1574-6968.2002.tb11460.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-12445650-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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