Article
High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus.
Gene - 1 Dec 1991
Lundberg K S, Shoemaker D D, Adams M W, Short J M, Sorge J A, Mathur E J
Abstract excerpt
A thermostable DNA polymerase which possesses an associated 3'-to-5' exonuclease (proofreading) activity has been isolated from the hyperthermophilic archaebacterium, Pyrococcus furiosus (Pfu). To test its fidelity, we have utilized a genetic assay that directly measures DNA polymerase fidelity i...
Topics
- Archaea
- Base Sequence
- Cloning, Molecular
- DNA-Directed DNA Polymerase
- Lac Operon
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Temperature