Article
High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus.
Gene - 1 Dec 1991
Lundberg K S, Shoemaker D D, Adams M W, Short J M, Sorge J A, Mathur E J
Abstract excerpt
A thermostable DNA polymerase which possesses an associated 3'-to-5' exonuclease (proofreading) activity has been isolated from the hyperthermophilic archaebacterium, Pyrococcus furiosus (Pfu). To test its fidelity, we have utilized a genetic assay that directly measures DNA polymerase fidelity in vitro during the polymerase chain reaction (PCR). Our results indicate that PCR performed with the DNA polymerase...
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