Article
Fidelity of DNA synthesis by the Thermococcus litoralis DNA polymerase--an extremely heat stable enzyme with proofreading activity.
Nucleic acids research - 25 Sept 1991
Mattila P, Korpela J, Tenkanen T, Pitkänen K
Abstract excerpt
We demonstrate that the DNA polymerase isolated from Thermococcus litoralis (VentTM DNA polymerase) is the first thermostable DNA polymerase reported having a 3'----5' proofreading exonuclease activity. This facilitates a highly accurate DNA synthesis in vitro by the polymerase. Mutational frequencies observed in the base substitution fidelity assays were in the range of 30 x 10(-6). These values were 5-10 times...
Topics
- Archaea
- DNA, Bacterial
- DNA-Directed DNA Polymerase
- Enzyme Stability
- Exodeoxyribonuclease V
- Exodeoxyribonucleases
- Hot Temperature
- Mutation
- Taq Polymerase
- Thermus