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Article

Fidelity of DNA synthesis by the Thermococcus litoralis DNA polymerase--an extremely heat stable enzyme with proofreading activity.

Nucleic acids research - 25 Sept 1991

Mattila P, Korpela J, Tenkanen T, Pitkänen K

Abstract excerpt

We demonstrate that the DNA polymerase isolated from Thermococcus litoralis (VentTM DNA polymerase) is the first thermostable DNA polymerase reported having a 3'----5' proofreading exonuclease activity. This facilitates a highly accurate DNA synthesis in vitro by the polymerase. Mutational frequencies observed in the base substitution fidelity assays were in the range of 30 x 10(-6). These values were 5-10 times...

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Identifiers

PMID: 1923765
DOI: 10.1093/nar/19.18.4967
PMCID: PMC328798

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