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Article

Enhancing the processivity of a family B-type DNA polymerase of Thermococcus onnurineus and application to long PCR.

Biotechnology letters - 1 May 2014

Kim Yun Jae, Lee Hyun Sook, Kwon Suk-Tae, Lee Jung-Hyun, Kang Sung Gyun

Abstract excerpt

Mechanisms that allow replicative DNA polymerases to attain high processivity are often specific to a given polymerase and cannot be generalised to others. Amplification efficiency is lower in family B-type DNA polymerases than in family A-type (Taq) polymerases because of their strong 3'-5' exonuclease-activity. Here, we have red the exonuclease domain of the Thermococcus onnurineus NA1 (TNA1) DNA polymerase,...

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PMID: 24375236
DOI: 10.1007/s10529-013-1441-x

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