Temperature effects on the fidelity of a thermostable HIV-1 reverse transcriptase.

Abstract excerpt

Transcriptomics and gene expression analysis are largely dependent of the availability of efficient thermostable reverse transcriptases (RTs). However, the intrinsic fidelity of DNA synthesis catalyzed by retroviral RTs is low. Reported error rates are in the range 1.2 × 10(-5)-6.7 × 10(-4), with oncoretroviral RTs being the most faithful enzymes. Wild-type HIV-1 group O (HIV-1O) RT is a thermostable polymerase...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Base Sequence
• Share to DNA Replication
• Share to HIV Reverse Transcriptase