Article
In vitro evolution of phi29 DNA polymerase using isothermal compartmentalized self replication technique.
Protein engineering, design & selection : PEDS - 1 Dec 2016
Povilaitis Tadas, Alzbutas Gediminas, Sukackaite Rasa, Siurkus Juozas, Skirgaila Remigijus
Abstract excerpt
Compartmentalized self replication (CSR) is widely used for in vitro evolution of thermostable DNA polymerases able to perform PCR in emulsion. We have modified and adapted CSR technique for isothermal DNA amplification using mezophilic phi29 DNA polymerase and whole genome amplification (WGA) reaction. In standard CSR emulsified bacterial cells are disrupted during denaturation step (94-96°C) in the first...
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