Article
High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase.
Nucleic acids research - 11 Jul 1990
Eckert K A, Kunkel T A
Abstract excerpt
We demonstrate that despite lacking a 3'----5' proofreading exonuclease, the Thermus aquaticus (Taq) DNA polymerase can catalyze highly accurate DNA synthesis in vitro. Under defined reaction conditions, the error rate per nucleotide polymerized at 70 degrees C can be as low as 10(-5) for base substitution errors and 10(-6) for frameshift errors. The frequency of mutations produced during a single round of DNA...
Topics
- Base Composition
- DNA, Bacterial
- DNA-Directed DNA Polymerase
- Hydrogen-Ion Concentration
- Magnesium Chloride
- Mutation
- Polymerase Chain Reaction
- Taq Polymerase
- Thermus