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Article

High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase.

Nucleic acids research - 11 Jul 1990

Eckert K A, Kunkel T A

Abstract excerpt

We demonstrate that despite lacking a 3'----5' proofreading exonuclease, the Thermus aquaticus (Taq) DNA polymerase can catalyze highly accurate DNA synthesis in vitro. Under defined reaction conditions, the error rate per nucleotide polymerized at 70 degrees C can be as low as 10(-5) for base substitution errors and 10(-6) for frameshift errors. The frequency of mutations produced during a single round of DNA...

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Identifiers

PMID: 2374708
DOI: 10.1093/nar/18.13.3739
PMCID: PMC331072

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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