Article
Fidelity of Thermococcus litoralis DNA polymerase (Vent) in PCR determined by denaturing gradient gel electrophoresis.
Nucleic acids research - 11 Aug 1991
Cariello N F, Swenberg J A, Skopek T R
Abstract excerpt
DNA synthesis fidelities of two thermostable DNA polymerases, Thermus aquaticus (Taq) and Thermococcus litoralis (Tli, also known as Vent), and a non-thermostable enzyme, a modified T7 DNA polymerase (Sequenase), were determined by analyzing polymerase chain reaction (PCR) products using denaturi...
Topics
- Base Sequence
- DNA Mutational Analysis
- DNA, Single-Stranded
- DNA-Directed DNA Polymerase
- Electrophoresis, Polyacrylamide Gel
- Humans
- Molecular Sequence Data
- Mutation
- Nucleic Acid Heteroduplexes
- Nucleic Acid Hybridization
- Polymerase Chain Reaction
- Taq Polymerase