Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Predominant mutations induced by the Thermococcus litoralis, vent DNA polymerase during DNA amplification in vitro.

PCR methods and applications - 1 May 1993

Keohavong P, Ling L, Dias C, Thilly W G

Abstract excerpt

We have analyzed the predominant mutations created during DNA amplification by PCR utilizing a DNA polymerase isolated from the Thermococcus litoralis (Vent DNA polymerase). Exon 3 of the human hypoxanthine guanine phosphoribosyl transferase (HPRT) gene was amplified using conditions optimized for efficiency of DNA amplification. The resulting PCR product was subjected to denaturing gradient gel electrophoresis...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Bacteria
Share to Base Sequence
Share to DNA

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to DNA-Directed DNA Polymerase

Share to Molecular Sequence Data

Share to Mutation

Share to Nucleic Acid Heteroduplexes

Share to Polymerase Chain Reaction

Source

Identifiers

PMID: 8324501
DOI: 10.1101/gr.2.4.288

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Predominant mutations induced by the Thermococcus litoralis, vent DNA polymerase during DNA amplification in vitro.PMID 8324501Read ↗

Fidelity of Thermococcus litoralis DNA polymerase (Vent) in PCR determined by denaturing gradient gel electrophoresis.Semantic proximity Read ↗

Fidelity of DNA synthesis by the Thermococcus litoralis DNA polymerase--an extremely heat stable enzyme with proofreading activity.Semantic proximity Read ↗

High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase.Semantic proximity Read ↗

Single molecule PCR in mtDNA mutational analysis: Genuine mutations vs. damage bypass-derived artifacts.Semantic proximity Read ↗

Screening for mutations in human HPRT cDNA using the polymerase chain reaction (PCR) in combination with constant denaturant gel electrophoresis (CDGE).Semantic proximity Read ↗

Evaluation of 15 polymerases and phosphorothioate primer modification for detection of UV-induced C:G to T:A mutations by allele-specific PCR.Semantic proximity Read ↗

Molecular diversity and catalytic activity of Thermus DNA polymerases.Semantic proximity Read ↗

Cold-sensitive mutants of Taq DNA polymerase provide a hot start for PCR.Semantic proximity Read ↗

The implications of using mutagenic primers in combination with Taq polymerase having proofreading activity.Semantic proximity Read ↗

DNA polymerase fidelity and the polymerase chain reaction.Semantic proximity Read ↗

Translesion synthesis across the (6-4) photoproduct and its Dewar valence isomer by the Y-family and engineered DNA polymerases.Semantic proximity Read ↗

In vitro evolution of phi29 DNA polymerase using isothermal compartmentalized self replication technique.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.