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Article

Coamplification at lower denaturation temperature-PCR increases mutation-detection selectivity of TaqMan-based real-time PCR.

Clinical chemistry - 1 Apr 2009

Li Jin, Wang Lilin, Jänne Pasi A, Makrigiorgos G Mike

Abstract excerpt

BACKGROUND: DNA genotyping with mutation-specific TaqMan(R) probes (Applied Biosystems) is broadly used in detection of single-nucleotide polymorphisms but is less so for somatic mutations because of its limited selectivity for low-level mutations. We recently described coamplification at lower denaturation temperature-PCR (COLD-PCR), a method that amplifies minority alleles selectively from mixtures of wild-type...

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Identifiers

PMID: 19233916
DOI: 10.1373/clinchem.2008.113381
PMCID: PMC2754313

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EGFR

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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