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Article

COLD-PCR-enhanced high-resolution melting enables rapid and selective identification of low-level unknown mutations.

Clinical chemistry - 1 Dec 2009

Milbury Coren A, Li Jin, Makrigiorgos G Mike

Abstract excerpt

BACKGROUND: Analysis of clinical samples often necessitates identification of low-level somatic mutations within wild-type DNA; however, the selectivity and sensitivity of the methods are often limiting. COLD-PCR (coamplification at lower denaturation temperature-PCR) is a new form of PCR that enriches mutation-containing amplicons to concentrations sufficient for direct sequencing; nevertheless, sequencing...

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Identifiers

PMID: 19815609
DOI: 10.1373/clinchem.2009.131029
PMCID: PMC2828872

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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