Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

PCR-based methods for the enrichment of minority alleles and mutations.

Clinical chemistry - 1 Apr 2009

Milbury Coren A, Li Jin, Makrigiorgos G Mike

Abstract excerpt

BACKGROUND: The ability to identify low-level somatic DNA mutations and minority alleles within an excess wild-type sample is becoming essential for characterizing early and posttreatment tumor status in cancer patients. Over the past 2 decades, much research has focused on improving the selectiv...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Nucleic Acid Denaturation
Share to Polymerase Chain Reaction
Share to Alleles

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Cancer Genomics and Diagnostics

Share to Molecular Biology Techniques and Applications

Share to Mutation

Share to Substrate Specificity

Share to Temperature

Source

Open in PubMed Open full text

Identifiers

PMID: 19201784
DOI: 10.1373/clinchem.2008.113035
PMCID: PMC2811432

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-19201784-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

PCR-based methods for the enrichment of minority alleles and mutations.PMID 19201784Read ↗

COLD-PCR: improving the sensitivity of molecular diagnostics assays.Semantic proximity Read ↗

Ice-COLD-PCR enables rapid amplification and robust enrichment for low-abundance unknown DNA mutations.Semantic proximity Read ↗

COLD-PCR: a new platform for highly improved mutation detection in cancer and genetic testing.Semantic proximity Read ↗

COLD-PCR Technologies in the Area of Personalized Medicine: Methodology and Applications.Semantic proximity Read ↗

Replacing PCR with COLD-PCR enriches variant DNA sequences and redefines the sensitivity of genetic testing.Semantic proximity Read ↗

COLD-PCR: Applications and Advantages.Semantic proximity Read ↗

Temperature-tolerant COLD-PCR reduces temperature stringency and enables robust mutation enrichment.Semantic proximity Read ↗

COLD-PCR enrichment of rare cancer mutations prior to targeted amplicon resequencing.Semantic proximity Read ↗

Nuclease-Assisted, Multiplexed Minor-Allele Enrichment: Application in Liquid Biopsy of Cancer.Semantic proximity Read ↗

COLD-PCR-enhanced high-resolution melting enables rapid and selective identification of low-level unknown mutations.Semantic proximity Read ↗

Coamplification at lower denaturation temperature-PCR increases mutation-detection selectivity of TaqMan-based real-time PCR.Semantic proximity Read ↗

Multiplex amplification coupled with COLD-PCR and high resolution melting enables identification of low-abundance mutations in cancer samples with low DNA content.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.