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Article

Enrichment of mutations in multiple DNA sequences using COLD-PCR in emulsion.

PloS one - 1 Jan 2012

Castellanos-Rizaldos Elena, Milbury Coren Audrey, Makrigiorgos G Mike

Abstract excerpt

BACKGROUND: Multiplex detection of low-level mutant alleles in the presence of wild-type DNA would be useful for several fields of medicine including cancer, pre-natal diagnosis and infectious diseases. COLD-PCR is a recently developed method that enriches low-level mutations during PCR cycling, thus enhancing downstream detection without the need for special reagents or equipment. The approach relies on the...

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PMID: 23236486
DOI: 10.1371/journal.pone.0051362
PMCID: PMC3516544

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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