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Article

High Efficiency Lipid-Based siRNA Transfection of Adipocytes in Suspension

10 Sept 2009

Abstract excerpt

BACKGROUND: Fully differentiated adipocytes are considered to be refractory to introduction of siRNA via lipid-based transfection. However, large scale siRNA-based loss-of-function screening of adipocytes using either electroporation or virally-mediated transfection approaches can be prohibitively complex and expensive. METHODOLOGY/PRINCIPAL FINDINGS: We present a method for introducing small interfering RNA...

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PMID: 19759827
DOI: 10.1371/journal.pone.0006940

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