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Article

Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases.

Proceedings of the National Academy of Sciences of the United States of America - 16 Dec 2008

Beumer Kelly J, Trautman Jonathan K, Bozas Ana, Liu Ji-Long, Rutter Jared, Gall Joseph G, Carroll Dana

Abstract excerpt

We report very high gene targeting frequencies in Drosophila by direct embryo injection of mRNAs encoding specific zinc-finger nucleases (ZFNs). Both local mutagenesis via nonhomologous end joining (NHEJ) and targeted gene replacement via homologous recombination (HR) have been achieved in up to 10% of all targets at a given locus. In embryos that are wild type for DNA repair, the products are dominated by NHEJ...

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Identifiers

PMID: 19064913
DOI: 10.1073/pnas.0810475105
PMCID: PMC2604940

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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