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Article

Targeted genome editing in pluripotent stem cells using zinc-finger nucleases.

Methods (San Diego, Calif.) - 1 Apr 2011

Bobis-Wozowicz Sylwia, Osiak Anna, Rahman Shamim H, Cathomen Toni

Abstract excerpt

Zinc-finger nucleases (ZFNs) are designer nucleases capable of cleaving a prespecified target DNA within complex genomes. ZFNs consist of a non-specific endonuclease domain fused to an engineered DNA-binding domain that tethers the nuclease activity to the chosen chromosomal site. The endonuclease-induced DNA double strand break triggers a cellular DNA damage response, resulting in double strand break repair by...

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Identifiers

PMID: 21185378
DOI: 10.1016/j.ymeth.2010.12.019

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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